The isolation of fetal cells from maternal circulation has the potential to allow relatively safe prenatal diagnosis for all pregnant women. The present technology, however, has not reached the accuracy required for clinical diagnosis because of
maternal cell contamination.
So we published a new method for enrichment of nRBC in a fetal cell isolation(1996).
In this study, attempted to FISH analysis of nRBC which was isolated by our own methods. We evaluated the efficiency of FISH.
As the results, we have successfully used FISH on enriched nRBC.
We were able to identified 2 abnormal fetus which were confirmed by conventional cytogenetic study as Down syndrome(Fig. 1) and Klinefeltre syndrome(Fig. 2). And the sensitivity and specificity for FISH was 86%(49/57) and 92.3%(36/39),
respectively.
According to our results, fetal cell analysis by FISH can be reliable used for prenatal aneuploidy diagnosis. However, the problems of enrichment of the fetal cell and FISH probe or condition should be over come before analyze.
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